A blocking ELISA for the detection of antibodies to psittacine beak and feather disease virus (BFDV)
نویسندگان
چکیده
منابع مشابه
A blocking ELISA for the detection of antibodies to psittacine beak and feather disease virus (BFDV).
Currently, the only diagnostic test available routinely for the sero-diagnosis of BFDV is the haemagglutination-inhibition (HI) assay. This test, whilst useful and applicable to samples from a wide range of psittacine birds, is not an ideal assay; it requires erythrocytes from live animals, virus purified from the feathers of infected birds and polyclonal antibody preparations in order to perfo...
متن کاملMolecular detection and characterization of beak and feather disease virus in psittacine birds in Tehran, Iran
Beak and feather disease virus (BFDV), a member of genus circovirus, is a small, non-enveloped, single stranded DNA virus. Although BFDVs are among the most well studied circoviruses, there is little to no information about BFDVs in Iran. The aim of the present study was to detect and identify BFDV molecules from the birds referred to the avian clinic of The Faculty of Veterinary Medicine, Tehr...
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Beak and feather disease is caused by Circovirus, which affects actively growing beak and feather cells of avian species. The disease affects mainly young birds while older birds may overcome the disease with few lasting effects. Due to lack of treatment, the only way to control the disease is through hygiene and early diagnosis. As a diagnostic tool, we have established a Taqman probe based re...
متن کاملDetection of Beak and Feather Disease Virus and Avian Polyomavirus Dna in Psittacine Birds in Poland
The first survey on the incidence of beak and feather disease virus (BFDV) and avian polyomavirus (APV) among the captive psittacine birds in Poland was presented in this study. Samples were collected between 2006 and 2009. A total number of 751 symptom–free birds from 31 genera and 59 species were tested for BFDV and APV by means of a PCR assay. BFDV was found in 25.3% and APV in 22.24% of the...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2009
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2009.02.009